Overview
- Peptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit CACNB3 (Accession P54286). Intracellular, C-terminus.
- Rat brain membranes (1:200-1:300).
Western blot analysis of rat brain membranes:1. Anti-CACNB3 Antibody (#ACC-008), (1:200).
2. Anti-CACNB3 Antibody, preincubated with CACNB3 Blocking Peptide (#BLP-CC008).
- Rat hippocampus and mouse cerebellum frozen sections.
- A7r5 cell line, derived from the rat thoracic aorta (1:400) (Uchida, R. et al. (2003) Eur. J. Pharmacol. 466, 53.).
The β3 protein is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genomes and they share some sequence as well as structure homology.1-3 The structure of CaVβ3 was recently determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficking to the plasma membrane as well as on current parameters such as voltage-dependent kinetics and interactions with modulatory proteins.1,2 CaVβ3 might interact with all the pore-forming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1
