Overview
- Peptide (C)DEVTDDYIGDNTTVD, corresponding to amino acid residues 26-40 of human CCR7 (Accession P32248). Extracellular, N-terminus.
- Jurkat, MOLT-4 and J774A.1 cells (1:200).
Western blot analysis of human T-cell leukemia Jurkat cell line (lanes 1 and 4), human acute lymphoblastic leukemia MOLT-4 (lanes 2 and 5) and mouse macrophage J774.1 (lanes 3 and 6) cell lysates:1-3. Anti-CCR7 (extracellular) Antibody (#ACR-027), (1:200).
4-6. Anti-CCR7 (extracellular) Antibody, preincubated with CCR7 (extracellular) Blocking Peptide (#BLP-CR027).
- Jurkat cells (5-10 µg/1x106 cells).
- The blocking peptide in not suitable for this application.
Chemokines (CHEMOtactic cytoKINES) are an important subgroup of the inflammatory cytokine family. More than fifty chemokines are expressed in mammalian cells and are characterized by their relatively small size (~70-90 amino acids), by their conserved N-terminus and cysteine motifs. This group of proteins has been further categorized on the basis of the cysteine spacing in the motifs creating C, CC, CXC, and CX3C chemokine subfamilies1,2.
All fifty chemokines exert their effects through twenty different chemokine receptors, belonging to the superfamily of G-protein coupled receptors (GPCRs) suggesting a certain level of promiscuity among the different receptors. All chemokine receptors couple to the pertussis sensitive Gi protein leading to phospholipase C activation and adenylate cyclase inhibition3.
Chemokines were first identified by their ability to mediate leukocyte chemoattraction. Apart from regulating the migration of leukocytes, they seem to be major players during inflammation and immunity4-6. Indeed, chemokines could also be further classified as being inflammatory as many chemokines are extensively upregulated in response to inflammation, or housekeeping important for the homeostasis of certain cell types. Inflammatory chemokines are responsible for recruiting immune cells to the inflamed region, and housekeeping chemokines, expressed in lymphoid or non-lymphoid tissues mediate the trafficking and targeting of cells7,8.
In general, chemokines and their receptors guide leukocytes to sites of infection/inflammation. However, cases of chronic inflammatory disease and tissue damage occur when there is excessive recruitment of leukocytes. They could also be involved in the pathogenesis of neurological diseases like multiple sclerosis and many inflammatory diseases like atherosclerosis and inflammatory bowel disease. Recently, chemokines and their receptors have been found to be involved in cancer metastasis, namely breast cancer1. The chemokine signaling also seems to be important for the communication between neural cells and the immune system, especially in the context of infection.
CCR7 receptor is responsible for directing dendritic cell migration to the lymph nodes where they are important for initiating the immune response. This receptor has two ligands, CCL19 and CCL21 which are expressed by stromal cells in the T cell-rich lymph node area9-11.
