Overview
- Peptide (C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2 (Accession P70673). Intracellular, C-terminus.
- Rat heart and mouse heart lysates. Human SHSY-5Y neuroblastoma cell lysate (1:200- 1:1000).
Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4):1-2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:500).
3-4. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
Western blot analysis of human SHSY-5Y neuroblastoma cell lysate:1. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:200).
2. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).
- Mouse hippocampal sections (1:300).
Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides.
The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle.
The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit.
The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes.
Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.
Multiplex staining of Kir6.2 and BDNF in mouse hippocampus.Immunohistochemical staining of immersion-fixed,free floating mouse brain frozen sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300) and Anti-BDNF Antibody (#ANT-010), (1:300). A. Kir6.2 staining (red) appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). B. BDNF staining (green) in the same section appears in the dentate gyrus granule layer (G) and in hilar interneurons (arrows). C. Merge of the two images reveals colocalization of Kir6.2 and BDNF in hippocampal interneurons.
