Overview
- Peptide GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1 (Accession Q63472). Intracellular, C-terminus.
- Rat brain lysate and HEK-293 cells transfected with KCNH1 (1:200). Mouse brain and colon lysates (Ousingsawat, J. et al. (2007) Clin. Cancer Res. 13, 824.).
Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4):1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).
3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).
- HEK 293T transfected cells (Hsu, P.H. et al. (2016) FEBS Open Bio 6, 349.).
- Paraffin embedded human gliomas (1:200) (Bai, Y. et al. (2013) Eur. J. Cancer 49, 710.).
- Paraformaldehyde-fixed human MDA-468 mamary gland adenocarcinoma cells (1:200).
KV10.1 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N- and C-termini.
The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.
Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.
