Overview
- Peptide (C)SAQRKKEMVLSEK, corresponding to amino acid residues 28-40 of human MAGT1 (Accession Q9H0U3). Extracellular, N-terminus.
Western blot analysis of mouse colon lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Western blot analysis of human HepG2 hepatocarcinoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and human
THP-1 monocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-MAGT1 (extracellular) Antibody (#ANT-197), (1:200).
4-6. Anti-MAGT1 (extracellular) Antibody, preincubated with MAGT1 (extracellular) Blocking Peptide (BLP-NT197).
Magnesium Transporter 1 (MAGT1) is a 335 amino acid peptide that is a part of the MAGT group of magnesium transporters. Magnesium (Mg2+) plays an essential cellular role, as a cofactor for ATP, nucleic acids, and numerous enzymes. It has been reported that it serves as a second messenger in intracellular signaling, and hence magnesium levels maintenance has a vast effect on cellular steady state 1.
In addition to its Mg2+ transporter activity, MAGT1 was also found to be a subunit of the oligosaccharyltransferase (OST) complex, through which it is responsible for protein glycosylation 2,3.
MAGT1 was shown to be related to the development of XMEN (X-linked immunodeficiency with magnesium defect, Epstein Barr Virus infection, and neoplasia) syndrome, for which Mg2+ supplementation has been shown to be beneficial. It is also related to atypical ATRX syndrome, intellectual disability and skin abnormalities.
Understanding the molecular basis of the involvement of magnesium and its channels in human pathogenesis can lead to the development of new medications for many diseases.
