Overview
- Peptide (C)KLENASWIHDPLMK, corresponding to amino acid residues 3-16 of rat NMUR2 (Accession Q9ESQ4). Extracellular, N-terminus.
- Rat and mouse brain, human brain glioblastoma (U-87 MG) and mouse brain glioma (C6) cells.
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), human brain glioblastoma (U-87 MG) (lanes 3 and 7) and mouse brain glioma (C6) cell (lanes 4 and 8) lysates:1-4. Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:200).
5-8. Anti-NMUR2 (extracellular) Antibody, preincubated with NMUR2 (extracellular) Blocking Peptide (#BLP-NR072).
Expression of Neuromedin U Receptor 2 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NMUR2 (extracellular) Antibody (#ANR-072), (1:100), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed immunoreactivity (green) in astrocytes (horizontal arrows), and neurons (vertical arrows). B. Pre-incubation of the antibody with NMUR2 (extracellular) Blocking Peptide (BLP-NR072), suppressed staining. Cell nuclei are stained with DAPI (blue).
- Rat C6 glioma cells (1:50).
Neuromedin U (NMU) is a highly conserved, ubiquitously distributed neuropeptide, which regulates food intake and body weight. Two high-affinity receptors have been identified for NMU: Neuromedin U receptor 1 (NMUR1) and Neuromedin U receptor 2 (NMUR2) that belong to the G-protein coupled receptors (GPCRs). These receptors are members of the rhodopsin-like class A GPCR family and share about 50% identity with each other in the seven transmembrane region.
NMUR2 is highly expressed in the central nervous system, in the paraventricular nucleus of the hypothalamus, nuclei accumbens, hippocampus, thalamus, medulla oblongata, and spinal cord. It is also detected in organs such as the uterus and ovary, and is abundant in nociceptive sensory pathways, including spinal dorsal horn, dorsal root ganglia, thalamus, and brain stem1-5.
Central effects of NMU, including the regulation of food intake, energy balance, stress response, and nociception, are mediated by NMUR2. In the brain, NMUR2 is predominant in the hypothalamic regions that are associated with regulation of food intake and energy balance5.
NMUR2 has been suggested as a potential target for developing new therapeutics for the treatment of eating disorders, obesity, stress-related disorders, and pain5.
Some studies have demonstrated that NMUR2 signaling in the paraventricular nucleus of the hypothalamus regulates consumption and preference for high-fat foods2.
