Overview
- Peptide (C)NKTD(S)RLENQLFIAKE, corresponding to amino acid residues 266 - 281 of mouse P2Y13 (Accession Q9D8I2). Extracellular, 3rd loop.
Western blot analysis of rat brain lysate (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat dorsal root ganglia lysate (lanes 3 and 7) and rat spleen lysate (lanes 4 and 8):1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 5), mouse BV-2 microglia cell line lysate (lanes 2 and 6), mouse J774 monocyte cell line lysate (lanes 3 and 7) and human HMC3 microglia cell line lysate (lanes 4 and 8):1-4. Anti-P2Y13 Receptor (extracellular) Antibody (#APR-103), (1:200).
5-8. Anti-P2Y13 Receptor (extracellular) Antibody, preincubated with P2Y13 Receptor (extracellular) Blocking Peptide (BLP-PR103).
The P2Y family belongs to the G-protein coupled receptors superfamily. They are activated by extracellular nucleotides and modulate variety of physiological functions.
A new member of this family was recently identified, the P2Y13 receptor.1,2 The P2Y13 receptor has significant similarity to the P2Y12 receptor (about 45% sequence identity) and together they form a distinct group structurally differ from other members of this family. P2Y13 receptor, like the P2Y12 receptor, is a high affinity receptor for the ADP nucleotide, coupled to the Gi class of G-proteins.1,2 The P2Y13 receptor is highly expressed at brain and spleen tissues as well as in the immune system. Lower expression was demonstrated in the testis, lung liver and other peripheral organs.1,2
Recently it has been shown that ADP activates a negative feedback pathway for ATP release from human red blood cells via the P2Y13 receptor.3
P2Y13 receptor was also implicated in inhibition of N-type Ca2+ channels in neurons, exerting pre and/or post-synaptic modulatory action.4
