Overview
- Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811). Intracellular, C-terminus.
- Rat brain membranes (1:200-1:300).
Western blot analysis of rat brain membranes:1. Anti-P2Y4 Receptor Antibody (#APR-006), (1:300).
2. Anti-P2Y4 Receptor Antibody, preincubated with P2Y4 Receptor Blocking Peptide (#BLP-PR006).
- HEK-293 transfected cells (1 μg) (Cavaliere, F. et al. (2004) Exp. Cell Res. 300, 149.).
- Rat brain frozen sections.
Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- Mouse macrophage J774 (Coutinho Silva, R. et al. (2005) Biochem. Pharmacol. 69, 641.).
- Rat osteoclasts (3 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The control antigen is not suitable for this application.
The P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
The P2Y4-receptor is highly expressed in the placenta and with lower levels in the lung and vascular smooth muscle.5
The human P2Y4 receptor is highly selective to UTP while rat P2Y4 receptor is equally activated by both UTP and ATP and also by other triphosphate nucleotides such as Ap4A.5
Recently it has been shown that P2Y4 receptor is involved in differentiation and cell death of human neuroblastoma SH-SY5Y cells.6
