Overview
- Peptide (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo2 (Accession Q9H5I5). The peptide is located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).
- Rat DRG lysates; mouse lung lysate; human HepG2 hepatocellular and HUVEC endothelial cell lysates (1:200-1:1000).
Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200).
3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).
Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.
Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains. Both channels open in response to mechanical stimuli, allowing positively charged ions, including calcium, to flow into the cell1.
Piezo2 protein contains over 2,800 residues; it forms a three-bladed, propeller like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1–36 (TM1–36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades2.
Piezo2 has an essential role in sensing gentle touch, tactile pain, airway stretch and lung inflation. It also serves as a baroreceptor for sensing blood pressure and regulating heart rate. Mutations in human Piezo2 are associated with distal arthrogryposis, a disorder characterized by joint deformities (contractures) that restrict movement in the hands and feet3,4.
