Overview
Cat #:
ANC-020
Alternative Name Protein RIC-3, RIC3 acetylcholine receptor chaperone, Resistant to inhibitor of cholinesterase 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide (C)EKLINRVGPNGESR, corresponding to amino acid residues 161-174 of mouse RIC3 (Accession Q8BPM6). Intracellular, C-terminus.
Accession (Uniprot) Number Q8BPM6
Gene ID 320360
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
RRID AB_2756679.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
- Mouse heart, rat heart and brain lysates. Human SH-SY5Y neuroblastoma cell lysate (1:200-1:1000).
Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:1. Anti-RIC3 Antibody (#ANC-020), (1:200).
2. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Western blot analysis of mouse heart membranes (lanes 1 and 4), rat heart lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6):1-3. Anti-RIC3 Antibody (#ANC-020), (1:200).
4-6. Anti-RIC3 Antibody, preincubated with RIC3 Blocking Peptide (#BLP-NC020).
Immunohistochemistry
- Mouse brain sections (1:400).
Scientific background
RIC-3 (Resistance to inhibitors of cholinesterase 3) is a conserved endoplasmic reticulum-resident protein that is known to be a chaperone for the nicotinic acetylcholine receptors (nAChR) and for the serotonin (5-HT3) channels1.
RIC-3 affects the maturation of multiple nAChRs which has been shown to facilitate nAChR assembly and trafficking3,4.
Multiple transcripts of RIC-3 displaying differential expression are detected in humans. The full-length transcript includes two transmembrane domains followed by a coiled-coil domain, a nonconserved N-terminus and C-terminus domain, which includes a second coiled-coil domain. The short isoform lacks the coiled-coil domains and is very conserved in mammals1,2,4.
Lyophilized Powder
For research purposes only, not for human use
Last Update: 03/01/2024
Specifications
Citations
Citations
