Overview
- Peptide (C)KGLVLGPIHKNQKDE, corresponding to amino acid residues 150 - 164 of mouse SLC3A2 (Accession P10852). Extracellular, C-terminus.
SLC3A2 (CD98) (extracellular) Blocking Peptide (#BLP-NT192)
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of mouse liver membranes (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:200).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Western blot analysis of human U-87 MG glioma cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):1-2. Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192), (1:400).
3-4. Anti-SLC3A2 (CD98) (extracellular) Antibody, preincubated with SLC3A2 (CD98) (extracellular) Blocking Peptide (BLP-NT192).
Solute carrier family 3 member 2 (SLC3A2), also known as 4F2 cell-surface antigen heavy chain (4F2HC) and CD98 antigen heavy chain (CD98hc), is a type II membrane protein belonging to the SLC3A/CD98 transporter family that plays a role in various physiological processes including amino acid transport, cell differentiation, migration, and proliferation.1,2,4
SLC3A2 is a type II glycosylated protein composed of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. SLC3A2 forms obligatory heterodimeric complexes with six members of the L-type amino acid transporters (LATs) SLC7A5-11, and SLC7A13.1
The heteromeric complex between SLC3A2 and SLC7A5 (also known as LAT1) is one of the best characterized. SLC7A5 is responsible for the transport of large neutral amino acids, including leucine, across the cell membrane. SLC3A2 acts as a chaperone protein for SLC7A5, stabilizing its expression on the cell surface and assisting in its proper functioning. This partnership is essential for amino acid transport, cell growth, and protein synthesis. This complex is particularly important in cancer cells and immune cells, as it facilitates nutrient uptake and influences cell proliferation and immune responses.1-4
In addition to SLC7A5, SLC3A2 also forms a complex with another SLC7 family member called SLC7A11 (also known as xCT). This complex is commonly referred to as the CD98/xCT or SLC3A2/SLC7A11.1-4
xCT, is a cystine/glutamate antiporter that plays a crucial role in the cellular exchange of cystine (an amino acid involved in the synthesis of the antioxidant glutathione) for glutamate. This exchange is important for maintaining cellular redox balance and protecting cells from oxidative stress.1,3,5
When SLC3A2 partners with SLC7A11, it facilitates the function of the xCT antiporter, allowing cells to import cystine while exporting glutamate. This process is essential for antioxidant defence and protecting cells from damage caused by reactive oxygen species (ROS). The SLC3A2/SLC7A11 complex has significant implications in cancer biology and the modulation of immune responses, as it can influence cellular redox status and affect processes related to cell proliferation and immune cell function.1,3,5
