Overview
- Peptide SDVDGTTYDFAHC, corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7). 3rd extracellular loop.
- LNCaP and DU145, human prostate carcinoma cell lines, mouse-TRPM8 transfected HEK-293 cells (1:200). It is recommended to add 0.5% Tween-20 to the antibody solution.
Western blot analysis of prostate carcinoma cell lines DU145 (lanes 1 and 3), Human LNCaP prostate carcinoma (lanes 2 and 4) and mouse-TRPM8 transfected HEK-293 (lanes 5 and 6) cell lysates:1,2,5. Anti-TRPM8 (extracellular) Antibody (#ACC-049), (1:200).
3,4,6. Anti-TRPM8 (extracellular) Antibody, preincubated with TRPM8 (extracellular) Blocking Peptide (#BLP-CC049).
- Transfected HEK-293 cells (Zeevi, D.A. et al. (2010) J. Cell Sci. 123, 3112.).
- Rat dorsal root ganglia (DRG) frozen section (1:100).
- Human prostate cancer DU145 cells (1:50) (Wang, Y. et al. (2012) Pathol. Oncol. Res. 18, 903.).
Cell surface detection of TRPM8 by indirect flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-TRPM8 (extracellular) Antibody (#ACC-049), (2.5μg) + goat-anti-rabbit-APC.
- Human, mouse and rat TRPM8 expressed in CHO cells, and rat DRGs (Miller, S. et al. (2014) PLoS ONE 9, e107151).
- Human live LNCaP cells (1:100). Rat live DRGs (1:500).
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1
The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.1
The TRPM8 channel is the cold and menthol receptor activated by either cold temperatures(~28°C) or menthol.2
TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5 Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6
Expression of TRPM8 in rat tail artery astrocytes.Immunocytochemical staining of rat tail artery astrocytes using Anti-TRPM8 (extracellular) Antibody (#ACC-049). TRPM8 staining is shown in green, nuclei are in blue. Omission of the antibody (lower panel) abolishes TRPM8 staining.Adapted from Johnson, C.D. et al. (2009) Am. J. Physiol. 296, H1868. with permission of the American Physiological Society.
