Overview
Cat #:
C-205
Lyophilized Powder yes
Source Synthetic
MW: 389.9
Purity: >99%
Form Lyophilized powder.
Effective concentration 1-100 µM.
Structure
Chemical name 6-Chloro-3,4-dihydro-3-(2-norbornen-5-yl)-2H-1,2-4-benzothiadiazine-7-sulfonamide 1,1-dioxide.
Molecular formula C14H16ClN3O4S2.
CAS No.: 2259-96-3
Activity Cyclothiazide is a selective AMPA receptor agonist (allosteric potentiator)1.
References-Activity
- Johansen, T.H. et al. (1995) Mol. Pharmacol. 48, 946.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage of solutions Up to four weeks at 4°C or three months at -20°C.
Our bioassay
Alomone Labs Cyclothiazide potentiates GluA1 channels expressed in Xenopus oocytes.Time course of current reversible potentiation by 1, 10 and 100 μM Cyclothiazide (#C-205) of glutamate-elicited currents (100 μM, holding potential -80 mV).
Scientific background Cyclothiazide (CTZ) is one of the most potent benzothiadiazides, a class of positive allosteric modulators of non-NMDA-type glutamate receptors, that inhibit receptor desensitization with a marked selectivity for the flip splice variants of AMPA receptors. Specifically, CTZ is known to produce a fast inhibition of AMPA receptor desensitization and a much slower potentiation of the AMPA current. By using patch-clamp techniques, the effects of CTZ were studied in HEK 293 cells stably transfected with the rat flip GluR1 subunit. The AMPA currents were greatly potentiated by CTZ in a dose-dependent manner. For instance, pretreatment of the cells with 100 μM CTZ increased the peak AMPA current 90-fold (EC50 = 28 μM), the current at the end of AMPA application 636-fold (EC50 = 46 μM), and the current integrals 730-fold (EC50 = 41 μM)1.
Target AMPA receptors
Lyophilized Powder
For research purposes only, not for human use
Last Update: 02/01/2024
Specifications
Citations
Citations
