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Home › Products › Ion Channels › Hyperpolarization-Activated Cation (HCN) Channels › Antibodies to HCN Channels

Certificate of Analysis

HCN Antibody Explorer Kit

A Screening Package of HCN Antibodies Economically Priced

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Cat #: AK-205
Size: 14 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-HCN1 Antibody Cat #: APC-056

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
Homology
Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.

HCN1 Blocking Peptide Cat #: BLP-PC056

Size: 40 µg

Guinea pig Anti-HCN1 Antibody Cat #: APC-056-GP (formerly AGP-203)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1 (Accession Q9JKB0). Intracellular, N-terminus.
Homology
Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical.

HCN1 Blocking Peptide Cat #: BLP-PC056

Size: 40 µg

Anti-HCN2 Antibody Cat #: APC-030

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

HCN2 Blocking Peptide Cat #: BLP-PC030

Size: 40 µg

Anti-HCN3 Antibody Cat #: APC-057

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3 (Accession Q9JKA8). Intracellular, C-terminus.
Homology
Human, mouse - identical.

HCN3 Blocking Peptide Cat #: BLP-PC057

Size: 40 µg

Anti-HCN3 (extracellular) Antibody Cat #: APC-083

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)ELEPRLDAEVYK, corresponding to amino acid residues 190-201 of rat HCN3 (Accession Q9JKA8). 2nd extracellular loop.
Homology
Mouse, human – identical.

HCN3 (extracellular) Blocking Peptide Cat #: BLP-PC083

Size: 40 µg

Anti-HCN4 Antibody Cat #: APC-052

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4). Intracellular, N-terminus.
Homology
Rabbit - identical; rat - 35/37 amino acid residues identical.

HCN4 Blocking Peptide Cat #: BLP-PC052

Size: 0.15 mg

Guinea pig Anti-HCN4 Antibody Cat #: APC-052-GP (formerly AGP-004)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
GST fusion protein with the sequence HGHLHDSAEE RRLIAEGDASPGEDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4 (Accession Q9Y3Q4). Intracellular, N-terminus.
Homology
Rabbit - identical; rat - 35/37 amino acid residues identical; mouse - 33/37 amino acid residues identical.

HCN4 Blocking Peptide Cat #: BLP-PC052

Size: 0.15 mg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#APC-056-GP & #APC-052-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use

Related Products

  1. Anti-HCN2-ATTO Fluor-594 Antibody (#APC-030-AR)
  2. Anti-PEX5L (TRIP8b) Antibody (#APR-070)
  3. Lidocaine (#L-105)

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