Overview
Cat #:
I-700
Lyophilized Powder yes
Origin Streptomyces conglobatus.
Source Natural
MW: 747.08
Purity: >99% (HPLC)
Effective concentration 100 nM - 2 µM.
Structure
Chemical name (4R,6S,8S,10Z,12R,14R,16E,18R,19R,20S,21S)-11,19,21-Tri hydroxy-4,6,8,12,14,18,20-heptamethyl-22-[(2S,2'R,5S,5'S)-octahydro-5'-[(1R)-1-hydroxyethyl]-2,5'-dimethyl[2,2'-bifuran]-5-yl]-9-oxo-10,16-docosadienoic acid calcium salt.
Molecular formula C41H70O9Ca.
CAS No.: 56092-82-1
Activity Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria1,2. In mammalian cells, Ionomycin acts as a selective Ca2+ ionophore3. In addition, Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells as well as apoptosis in various cell lines4-6.
References-Activity
- Liu, W.C. et al. (1978) J. Antibiot. (Tokyo) 31, 815.
- Liu, C. et al. (1978) J. Biol. Chem. 253, 5892.
- Kauffman, R.F. (1980) J. Biol. Chem. 255, 2735.
- Chatila, T. et al. (1989) J. Immunol. 143, 41283.
- Takei, N. et al. (1994) Brain Res. 652, 65.
- Aagaard-Tillery, K.M. and Jelinek D.F. (1995) J. Immunol. 155, 3297.
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO or methanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to two week at 4°C or three months at -20°C.
Protect from light.
Protect from light.
Our bioassay
Alomone Labs Ionomycin calcium salt induces cytosolic Ca2+ increase.Ca2+ traces from Fluo-3 AM loaded C6 glioma cells treated with 1 µM Ionomycin calcium salt (#I-700).
Scientific background
Ionomycin is a narrow spectrum antibiotic for gram-positive bacteria produced by Streptomyces conglobatus. In mammalian cells, ionomycin acts as a potent and selective Ca2+ ionophore, more effective than A23187. The Ca2+ influx by ionomycin is caused by direct stimulation of store-regulated cation entry and not by direct action at the plasma membrane.1-4
Ionomycin induces hydrolysis of phosphoinositides, activation of PKC in human T cells and apoptosis in various cells lines.5-7
Lyophilized Powder
Image & Title 
Alomone Labs Ionomycin calcium salt increases [Ca2+]i in mouse sperm.Mouse sperm were incubated under capacitating conditions (CAP) and treated with soluble zona pellucida (sZP), which caused an increase in [Ca2+]i. Administration of Ionomycin calcium salt (#I-700) caused an additional increase in [Ca2+]i.Adapted from Wertheimer, E. et al. (2013) J. Biol. Chem. 288, 35307. with permission of The American Society for Biochemistry and Molecular Biochemistry.
Alomone Labs Ionomycin calcium salt increases [Ca2+]i in mouse sperm.Mouse sperm were incubated under capacitating conditions (CAP) and treated with soluble zona pellucida (sZP), which caused an increase in [Ca2+]i. Administration of Ionomycin calcium salt (#I-700) caused an additional increase in [Ca2+]i.Adapted from Wertheimer, E. et al. (2013) J. Biol. Chem. 288, 35307. with permission of The American Society for Biochemistry and Molecular Biochemistry.
For research purposes only, not for human use
Last Update: 02/01/2024
Specifications
Citations
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