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Home › Products › Ion Channels › Na+ Channels › Voltage-Gated Na+ Channels › Antibodies to NaV Channels

Certificate of Analysis

NaV1.8 Channel Basic Research Pack

All You Need for NaV1.8 Channel Research

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Cat #: ESB-003
Size: 5 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: M, R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.

Anti-NaV1.8 (SCN10A) (extracellular) Antibody Cat #: ASC-028

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EDNYLEEKPRVKSVLE, corresponding to amino acid residues 1173-1188 of rat NaV1.8 (Accession Q62968). 1st extracellular loop, domain III.
Homology
Mouse - identical; human – Not recommended for use with human samples.

Pharmacological Reagents

A-803467 Cat #: A-105_50 mg

Size: 50 mg Source: Synthetic MW: 357.8 Purity: >99% (HPLC)
Effective concentration
10 nM - 10 μM.
Activity
A-803467 is a potent and selective blocker of the TTX-resistant NaV1.8 channel. A-803467 is a state dependent inhibitor with IC50 of 8 nM at depolarized membrane potential (-40 mV) and 80 nM at -100 mV. As an efficient inhibitor of TTX-resistant currents in DRG neurons, A-803467 was also effective as an analgesic in several rodent models of different pathological pain states1,2. A-803467 was used as a pharmacological tool to demonstrate the expression of NaV1.8 in the heart conduction system and its contribution to QRS interval in the mouse heart3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.
For research purposes only, not for human use

Related Products

  1. NaV1.8 Channel Premium Research Pack (#ESP-003)
  2. NaV1.8 Channel Deluxe Research Pack (#ESD-003)
  3. Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR)
  4. Guinea pig Anti-NaV1.8 (SCN10A) Antibody (#ASC-016-GP (formerly AGP-029))
  5. Anthopleurin-C (#A-400)
  6. Jingzhaotoxin-II (#STJ-150)
  7. Pterinotoxin-1 (#STT-100)
  8. VSTX3 (#STT-350)

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