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Home › Products › Ion Channels › Ligand-Gated Ion Channels › Purinergic (P2X) Receptors › Antibodies to P2X Receptors

Certificate of Analysis

P2X Receptor Antibody Explorer Kit

A Screening Package of P2X Receptor Antibodies Economically Priced

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Cat #: AK-201
Size: 28 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X1 Receptor Antibody Cat #: APR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 receptor (Accession P47824). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.

P2X1 Receptor Blocking Peptide Cat #: BLP-PR001

Size: 40 µg

Anti-P2X1 Receptor (extracellular) Antibody Cat #: APR-022

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CRPIYEFHGLYEEK, corresponding to amino acid residues 270-283 of human P2X1 receptor (Accession P51575). Extracellular loop.
Homology
Rat, mouse - 11/14 amino acid residues identical.

P2X1 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR022

Size: 40 µg

Anti-P2X2 Receptor Antibody Cat #: APR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)SQQDSTSTDPKGLAQL, corresponding to amino acid residues 457-472 of rat P2X2 receptor (Accession P49653). Intracellular, C-terminus.
Homology
Guinea pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.

P2X2 Receptor Blocking Peptide Cat #: BLP-PR003

Size: 40 µg

Anti-P2X2 Receptor (extracellular) Antibody Cat #: APR-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KSDYLKHSTFDQDS, corresponding to amino acid residues 207-220 of rat P2X2 with replacement of cysteine 214 (C214) with serine (*S) (Accession P49653). Extracellular.
Homology
Rat, mouse - 13/14 amino acid residues identical.
Will not recognize human P2X2.

P2X2 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR025

Size: 40 µg

Anti-P2X3 Receptor Antibody Cat #: APR-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 receptor (Accession P49654). Intracellular, C-terminus.
Homology
Mouse - identical; human - 13/15 amino acid residues identical.

P2X3 Receptor Blocking Peptide Cat #: BLP-PR016

Size: 40 µg

Anti-P2X3 Receptor (extracellular) Antibody Cat #: APR-026

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Homology
Mouse – identical; human - 13/15 amino acid residues identical.

P2X3 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR026

Size: 40 µg

Anti-P2X4 Receptor Antibody Cat #: APR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 receptor (Accession P51577). Intracellular, C-terminus.
Homology
Mouse - identical; human - 15/19 amino acid residues identical; chicken - 11/12 amino acid residues identical; Xenopus - 11/11 amino acid residues identical.

P2X4 Receptor Blocking Peptide Cat #: BLP-PR002

Size: 40 µg

Anti-P2X4 Receptor (extracellular) Antibody Cat #: APR-024

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)RDLAGKEQRTLTK, corresponding to amino acid residues 301-313 of rat P2X4 receptor (Accession P51577). Extracellular.
Homology
Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

P2X4 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR024

Size: 40 µg

Anti-P2X5 Receptor Antibody Cat #: APR-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, WB Reactivity: R
Immunogen
Peptide (C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 of rat P2X5 receptor (Accession P51578). Intracellular, C-terminus.
Homology
Mouse - 13/18 amino acid residues identical.

P2X5 Receptor Blocking Peptide Cat #: BLP-PR005

Size: 40 µg

Anti-P2X5 Receptor (extracellular) Antibody Cat #: APR-027

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor (Accession P51578).
Homology
Mouse –12/13 amino acid residues; human – 10/13 amino acid residues.

P2X5 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR027

Size: 40 µg

Anti-P2X6 Receptor Antibody Cat #: APR-013

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RTKYEEARAPKATTNSA, corresponding to amino acid residues 363-379 of rat P2X6 receptor (Accession P51579). Intracellular, C-terminus.
Homology
Mouse - 15/17 amino acid residues identical; human - 14/17 amino acid residues identical.

P2X6 Receptor Blocking Peptide Cat #: BLP-PR013

Size: 60 µg

Anti-P2X6 Receptor (extracellular) Antibody Cat #: APR-028

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide CPEGEMGTYSHGIK, corresponding to amino acid residues 136-149 of rat P2X6 receptor (Accession P51579). Extracellular.
Homology
Mouse - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.

P2X6 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR028

Size: 40 µg

Anti-P2X7 Receptor Antibody Cat #: APR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Homology
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.

P2X7 Receptor Blocking Peptide Cat #: BLP-PR004

Size: 40 µg

Anti-P2X7 Receptor (extracellular) Antibody Cat #: APR-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Homology
Human, rat - identical; bovine - 14/17 amino acid residues identical.

P2X7 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR008

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use

Related Products

  1. Anti-P2X1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-022-AG)
  2. Anti-P2X1 Receptor (extracellular)-FITC Antibody (#APR-022-F)
  3. Anti-P2X2 Receptor-ATTO Fluor-594 Antibody (#APR-003-AR)
  4. Anti-P2X3 Receptor-ATTO Fluor-594 Antibody (#APR-016-AR)
  5. Guinea pig Anti-P2X4 Receptor Antibody (#APR-002-GP (formerly AGP-085))
  6. Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO)
  7. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG)
  8. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR)
  9. Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F)
  10. P2X Receptor Antibodies for Pain Research Explorer Kit (#AK-380)
  11. Extracellular-Targeted P2X Receptor Antibody Explorer Kit (#AK-480)
  12. Purinergic Receptor Antibodies for Pain Research Explorer Kit (#AK-400)
  13. BomoTx Toxin (#NTB-200)
  14. Garcinolic acid (#G-155)
  15. P2X7 Receptor Antagonist Explorer Kit (#EK-410)
  16. A-317491 sodium salt hydrate (#A-380)
  17. Evans Blue tetrasodium salt (#E-110)
  18. RO-3 (#R-190)

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