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Home › Products › Ion Channels › ASIC/ENaC/Degenerin Family › Acid-Sensing Ion Channels (ASIC) › Antibodies to ASICs

Certificate of Analysis

Pain-Related DRG Marker Antibody Kit

A Package of Pain-Related DRG Marker Antibodies Economically Priced

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Cat #: AK-550
Size: 18 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPA1 (extracellular) Antibody Cat #: ACC-037

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.
Homology
Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.

TRPA1 (extracellular) Blocking Peptide Cat #: BLP-CC037

Size: 40 µg

Anti-TRPV1 (VR1) Antibody Cat #: ACC-030

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EDAEVFKDSMVPGEK, corresponding to amino acid residues 824-838 of rat TRPV1 (Accession O35433). Intracellular, C-terminus.
Homology
Mouse, guinea pig - 14/15 amino acid residues identical; human - 10/15 amino acid residues identical.

TRPV1/VR1 Blocking Peptide Cat #: BLP-CC030

Size: 40 µg

Anti-NaV1.7 (SCN9A) Antibody Cat #: ASC-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Homology
Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.

Nav1.7/SCN9A Blocking Peptide Cat #: BLP-SC008

Size: 40 µg

Guinea pig Anti-NaV1.8 (SCN10A) Antibody Cat #: ASC-016-GP (formerly AGP-029)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)EDEVAAKEGNSPGPQ, corresponding to amino acid residues 1943-1956 of rat NaV1.8 (Accession Q63554). Intracellular, C-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 7/15 amino acid residues identical.

Nav1.8/SCN10A Blocking Peptide Cat #: BLP-SC016

Size: 40 µg

Guinea pig Anti-ASIC3 Antibody Cat #: ASC-018-GP (formerly AGP-052)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

ASIC3 Blocking Peptide Cat #: BLP-SC018

Size: 40 µg

Anti-P2X3 Receptor (extracellular) Antibody Cat #: APR-026

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
Homology
Mouse – identical; human - 13/15 amino acid residues identical.

P2X3 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR026

Size: 40 µg

Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody Cat #: AOR-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)ELVPSARAELQSSPLVN corresponding to amino acid residues 2-18 of mouse δ-Opioid receptor (Accession P32300). Extracellular, N-terminus.
Homology
Rat - 13/17 amino acid residues identical.

δ-Opioid Receptor/OPRD1 (extracellular) Blocking Peptide Cat #: BLP-OR014

Size: 40 µg

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody Cat #: AOR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of the rat µ-Opioid receptor (Accession P33535). Extracellular, N-terminus.
Homology
Mouse - identical; human - 15/17 amino acid residues identical.

µ-Opioid Receptor/OPRM1 (extracellular) Blocking Peptide Cat #: BLP-OR011

Size: 40 µg

Anti-TrkA (extracellular) Antibody Cat #: ANT-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). 2nd extracellular immunoglobulin-like domain.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

TrkA (extracellular) Blocking Peptide Cat #: BLP-NT018

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#ASC-016-GP & #ASC-018-GP) are included in this Market Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use

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