Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc
For research purposes only, not for human use
Properties
Sequence
- (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession P25427).
Accession (Uniprot) Number P25427
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Recombinant mouse proNGF protein (#N-250), (lanes 1-3) and Recombinant human proNGF protein (#N-280), (lanes 4-6):1,4. 100 ng + Anti-proNGF Antibody (ANT-005), (1:200).
2,5. 250 ng + Anti-proNGF Antibody (1:200).
3,6. 500 ng + Anti-proNGF Antibody (1:200).
Western blot analysis of mouse submandibular glands (SMGs):1. Anti-proNGF Antibody (#ANT-005), (1:200).
2. Anti-proNGF Antibody, preincubated with proNGF Blocking Peptide (#BLP-NT005).- Rat brain sections.
Western blot analysis of 100 ng Recombinant mouse proNGF protein (#N-250) (lanes 1 and 3) and Recombinant human proNGF protein (#N-280) (lanes 2 and 4):1-2. Guinea pig Anti-proNGF Antibody (#ANT-005-GP), (1:200).
3-4. Guinea pig Anti-proNGF Antibody, preincubated with the blocking peptide.- Mouse brain sections (1:400).
Last update: 02/01/2024
