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Home › Products › Ion Channels › Renal Membrane Proteins › Antibodies

Certificate of Analysis

Renal Purinergic Receptor Antibody Explorer Kit

A Screening Package of Renal Purinergic Receptor Antibodies Economically Priced

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Cat #: AK-645
Size: 16 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-P2X7 Receptor Antibody Cat #: APR-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
Homology
Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.

P2X7 Receptor Blocking Peptide Cat #: BLP-PR004

Size: 40 µg

Anti-P2Y1 Receptor Antibody Cat #: APR-009

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1. (Accession P49651, P47900). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.

P2Y1 Receptor Blocking Peptide Cat #: BLP-PR009

Size: 40 µg

Anti-P2Y2 Receptor Antibody Cat #: APR-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KPAY GTTGL PRAKR KSVR, corresponding to residues 227-244 of rat P2RY2 (Accession P41232). 3rd intracellular loop (i3) between TM5 and TM6 domains.
Homology
Mouse - 18/19 amino acid residues identical; human - 17/19 amino acid residues identical.

P2Y2 Receptor Blocking Peptide Cat #: BLP-PR010

Size: 40 µg

Anti-P2Y4 Receptor Antibody Cat #: APR-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2RY4 (Accession O35811). Intracellular, C-terminus.
Homology
Mouse - 13/14 amino acid residues identical.

P2Y4 Receptor Blocking Peptide Cat #: BLP-PR006

Size: 40 µg

Anti-P2Y6 Receptor Antibody Cat #: APR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.

P2Y6 Receptor Blocking Peptide Cat #: BLP-PR011

Size: 40 µg

Anti-Adenosine A1 Receptor Antibody Cat #: AAR-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR (Accession P30542). 3rd intracellular loop.
Homology
Mouse, rat, bovine, canis and pig - identical.

Adenosine A1 Receptor Blocking Peptide Cat #: BLP-AR006

Size: 40 µg

Anti-Adenosine A2A Receptor Antibody Cat #: AAR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor (Accession Q60613). 3rd intracellular loop.
Homology
Rat, human, and canis – identical.

Adenosine A2A Receptor Blocking Peptide Cat #: BLP-AR002

Size: 40 µg

Anti-Adenosine A2B Receptor (extracellular) Antibody Cat #: AAR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). 2nd extracellular loop.
Homology
Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical.

Adenosine A2B Receptor (extracellular) Blocking Peptide Cat #: BLP-AR003

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use

Related Products

Antibodies

  1. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008)
  2. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-633 Antibody (#APR-008-FR)
  3. Anti-P2X7 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-008-AG)
  4. Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F)
  5. Anti-P2X7 Receptor-ATTO Fluor-550 Antibody (#APR-004-AO)
  6. Anti-Adenosine A2A Receptor (extracellular) Antibody (#AAR-008)
  7. Anti-Human Adenosine A2A Receptor (extracellular) Antibody (#AAR-007)
  8. Anti-Human Adenosine A2A Receptor (extracellular)-FITC Antibody (#AAR-007-F)
  9. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021)
  10. Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-021-AG)
  11. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102)
  12. Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F)

Pharmacological tools

Blockers/Antagonists: small molecules
  1. A-740003 (#A-420)
  2. A-804598 (#A-415)
  3. A-839977 (#A-470)
  4. AACBA hydrochloride (#A-410)
  5. AZ 10606120 dihydrochloride (#A-405)
  6. AZ11645373 (#A-395)
  7. AZD-9056 hydrochloride (#A-425)
  8. GW 791343 hydrochloride (#G-115)
  9. JNJ-47965567 (#J-115)
  10. JNJ-54166060 (#J-125)
  11. KN-62 (#K-120)
  12. ZINC9315614 (#Z-145)
Activators/Agonists: small molecules
  1. BzATP triethylammonium salt (#A-385)
  2. Garcinolic acid (#G-155)

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