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Home › Products › Ion Channels › TRP Channels › TRPC Channels › Antibodies to TRPC Channels

Certificate of Analysis

TRPC Channel Antibody Explorer Kit

A Screening Package of TRPC Channel Antibodies Economically Priced

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Cat #: AK-212
Size: 24 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-TRPC1 Antibody Cat #: ACC-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). Intracellular.
Homology
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.

TRPC1 Blocking Peptide Cat #: BLP-CC010

Size: 40 µg

Anti-TRPC1 (extracellular) Antibody Cat #: ACC-118

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)SMGQMLQDFGK, corresponding to amino acid residues 495-505 of rat TRPC1 (Accession Q9QX01). 2nd extracellular loop.
Homology
Human, mouse - identical.

TRPC1 (extracellular) Blocking Peptide Cat #: BLP-CC118

Size: 40 µg

Anti-TRPC2 Antibody Cat #: ACC-027

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)KNRSGVRMFKDGD, corresponding to amino acid residues 12-24 of mouse TRPC2 (Accession Q9R244). Intracellular, N-terminus.
Homology
Rat - 12/13 amino acid residues identical.

TRPC2 Blocking Peptide Cat #: BLP-CC027

Size: 40 µg

Anti-TRPC3 Antibody Cat #: ACC-016

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Homology
Human, rat - 13/14 amino acid residues identical.

TRPC3 Blocking Peptide Cat #: BLP-CC016

Size: 40 µg

Guinea pig Anti-TRPC3 Antibody Cat #: ACC-016-GP (formerly AGP-018)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide HKLSEKLNPSVLRC, corresponding to amino acid residues 822-835 of mouse TRPC3 (Accession Q9QZC1). Intracellular, C-terminus.
Homology
Human, rat - 13/14 amino acid residues identical.

TRPC3 Blocking Peptide Cat #: BLP-CC016

Size: 40 µg

Anti-TRPC4 Antibody Cat #: ACC-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession Q9QUQ5). Intracellular, C-terminus.
Homology
Rat, human - 15/16 amino acid residues identical; bovine -  11/15 amino acid residues identical.

TRPC4 Blocking Peptide Cat #: BLP-CC018

Size: 40 µg

Anti-TRPC4 (extracellular) Antibody Cat #: ACC-119

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KYSALNPRESWD, corresponding to amino acid residues 458-469 of rat TRPC4 (Accession O35119). 2nd extracellular loop.
Homology
Mouse, human - identical.

TRPC4 (extracellular) Blocking Peptide Cat #: BLP-CC119

Size: 40 µg

Anti-TRPC5 Antibody Cat #: ACC-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)HKWGDGQEEQVTTRL, corresponding to amino acid residues 959-973 of human TRPC5 (Accession Q9UL62). Intracellular, C-terminus.
Homology
Rat, mouse, rabbit - identical.

TRPC5 Blocking Peptide Cat #: BLP-CC020

Size: 40 µg

Anti-TRPC6 Antibody Cat #: ACC-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.

TRPC6 Blocking Peptide Cat #: BLP-CC017

Size: 40 µg

Guinea pig Anti-TRPC6 Antibody Cat #: ACC-017-GP (formerly AGP-002)

Size: 50 µl Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.

TRPC6 Blocking Peptide Cat #: BLP-CC017

Size: 40 µg

Anti-TRPC6 (extracellular) Antibody Cat #: ACC-120

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.
Homology
Rat, human - identical

TRPC6 (extracellular) Blocking Peptide Cat #: BLP-CC120

Size: 40 µg

Anti-TRPC7 (extracellular) Antibody Cat #: ACC-066

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DQHVQDDTLHNVS, corresponding to amino acid residues 504-516 of human TRPC7 (Accession Q9HCX4). 2nd extracellular loop.
Homology
Rat, mouse - 11/13 amino acid residues identical.

TRPC7 (extracellular) Blocking Peptide Cat #: BLP-CC066

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

Note
Guinea pig polyclonal antibodies (#ACC-016-GP & #ACC-017-GP) are included in this Explorer Kit. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use

Related Products

Antibodies

  1. Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR)

Pharmacological tools

Blockers/Antagonists: peptides/peptide toxins
  1. GsMTx-4 (#STG-100)
Blockers/Antagonists: small molecules
  1. SKF 96365 hydrochloride (#S-175)

Explorer kits & Research packs

Explorer kits
  1. Mechanosensitive TRP Channel Antibody Explorer Kit (#AK-305)
  2. pH-Sensitive TRP Channel Antibody Explorer Kit (#AK-475)

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