Overview
Type: Synthetic peptide
Form: Lyophilized powder
CCKBR (extracellular) Blocking Peptide (#BLP-CR042) is the original antigen used for immunization during Anti-CCKBR (extracellular) Antibody (#ACR-042) generation. The blocking peptide binds and ‘blocks’ Anti-CCKBR (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:1,2. Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200).
3,4. Anti-CCKBR (extracellular) Antibody, preincubated with CCKBR (extracellular) Blocking Peptide (#BLP-CR042).
Expression of Cholecystokinin B receptor in rat stomachImmunohistochemical staining of paraffin embedded rat stomach sections using Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:100). Cholecystokinin B receptor (brown) is expressed in both parietal cells (black arrows) and in chief cells (red arrows) of the gastric mucosa. Hematoxilin is used as the counterstain.
Expression of CCKBR in rat hypothalamus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat dorsomedial hypothalamus region, showed immunoreactivity (green) in neurons (vertical arrows) and along the wall of 3rd ventricle (horizontal arrow). B. Pre-incubation of the antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue). 3rd V = Third ventricle.
Expression of CCKBR in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-CCKBR (extracellular) Antibody (#ACR-042), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity (green) in the granule layer (G, vertical arrows) and in interneurons (horizontal arrows). B. Pre-incubation of the antibody with CCKBR (extracellular) Blocking Peptide (#BLP-CR042), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- CETPRIRGTGTRELE, corresponding to amino acid residues 39-53 of mouse Gastrin/cholecystokinin type B receptor (Accession P56481).
Accession (Uniprot) Number P56481
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
