Overview
Type: Synthetic peptide
Form: Lyophilized powder
GDNF Blocking Peptide (#BLP-NT014) is the original antigen used for immunization during Anti-GDNF Antibody (#ANT-014) generation. The blocking peptide binds and ‘blocks’ Anti-GDNF primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of Recombinant human GDNF protein:Lanes 1, 4: 50 ng Recombinant human GDNF protein (#G-240).
Lanes 2, 5: 10 ng Recombinant human GDNF protein.
Lanes 3, 4: 1 ng Recombinant human GDNF protein.
Lanes 1-3: Anti-GDNF Antibody (#ANT-014), (1:1000).
Lanes 4-5: Anti-GDNF Antibody, preincubated with GDNF Blocking Peptide (#BLP-NT014).
Expression of GDNF in rat dorsal root ganglion (DRG).Immunohistochemical staining of rat DRG longitudinal frozen section using Anti-GDNF Antibody (#ANT-014), (1:50), followed by Alexa 555-conjugated goat anti-rabbit antibody (red). Hoechst 33342 (blue) was used as a counterstain. GDNF staining is specific for neurons. Note that Schwann cells as well as satellite glial cells are both negative.
Expression of GDNF in rat hippocampusImmunohistochemical staining of rat hippocampus frozen sections with Anti-GDNF Antibody (#ANT-014), (1:100), (red). A. GDNF staining is apparent in the pyramidal layer (asterik), in astrocytic fibers (horizontal arrows) and in some interneurons (vertical arrow). B. Calbindin D28k (green) appears in a subset of cells in the pyramidal layer. C. Merge of GDNF and calbindin demonstrates some co-localization in neurons. DAPI is used as the counterstain (blue).
Properties
Sequence
- (C)DDNLVYHILRKHSAKR, corresponding to amino acid residues 192-207 of human GDNF (precursor) (Accession P39905).
Accession (Uniprot) Number P39905
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
