Overview
Type: Synthetic peptide
Form: Lyophilized powder
TrkA (extracellular) Blocking Peptide (#BLP-NT018) is the original antigen used for immunization during Anti-TrkA (extracellular) Antibody (#ANT-018) generation. The blocking peptide binds and ‘blocks’ Anti-TrkA (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-TrkA (extracellular) Antibody (#ANT-018), (1:200).
3,4. Anti-TrkA (extracellular) Antibody, preincubated with TrkA (extracellular) Blocking Peptide (#BLP-NT018).
Expression of TrkA in rat DRGImmunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:100). TrkA (red staining) is expressed in DRG neurons and in satellite microglia (arrows). Hoechst 33342 is used as the counterstain.
Expression of TrkA in rat brain hippocampal dentate gyrusImmunohistochemical staining of immersion-fixed, free floating rat brain frozen sections. A. Brain sections were stained using Anti-TrkA (extracellular) Antibody (#ANT-018), (1:1000), (green staining). B. The same section was also stained for glial fibrillary acidic protein (GFAP) (red and counterstained blue). C. Overlay of A and B demonstrates co-localization of TrkA and GFAP in dentate gyrus astrocytes.
Properties
Sequence
- (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739).
Accession (Uniprot) Number P35739
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
